Plant Biotechnology

Contents

1. History of Plant Biotechnology

1.1 History and Definition

1.2 Concept of Biotechnology

1.3 Scope and Importance of plant Biotechnology

1.4 Multidisciplinary Nature of Biotechnology

1.5 Current status of Biotechnology in India

1.6 Biotechnology Research in India

2. Plant Tissue Culture

2.1 Concept of Tissue Culture and Cellular Totipotency

2.2 Basic Techniques

2.2.1 Selection of Explants

2.2.2 Types of Culture

2.2.3 Requirements of Plant Tissue Culture

2.2.4 Steps in Plant Tissue Culture

2.2.5 Sterilization of Explant and Isolation

2.2.6 Inoculation/Transfer of Explants

2.2.7 Incubation

2.2.8 Subculture

2.2.9 Hardening

2.2.10 Maintenance of Aseptic Environment /Sterilization

2.3 Application of Micropropagation

2.4 Commercial Plant Tissue Culture Laboratories in Maharashtra and India

3. Single Cell Protein

3.1 Concept and Definition

3.2 Importance of Proteins in Diet

3.3 Production of SCP from Spirulina and Yeast

3.3.1 SCP from Spirulina (Algae)

3.3.2 SCP from Yeast

3.4 Importance and Acceptability of SCP

3.4.1 Importance of SCP

3.4.2  Acceptability of SCP

4. Plant Genetic Engineering

4.1 Introduction, Concept

4.2 Tools of Genetic Engineering (Restriction Enzymes, Ligases, Plasmid Vectors)

4.2.1 Restriction Enzymes (Restriction Endonucleases)

4.2.2 Ligases

4.2.3 Cloning Vector

4.3 Gene Cloning Technique

4.3.1 Direct DNA Transfer

4.3.2 Vector Mediated Gene Transfer

4.4 Application of Genetic Engineering; Insect Pest Resistance, Abiotic Stress Tolerance, Herbicide Resistance

4.4.1 Insect Pest Resistance

4.4.2 Abiotic Stress Tolerance

4.4.3 Herbicide resistance

5. Genomics, Proteomics and Bioinformatics

5.1 Genomics – Definition and Concept

5.1.1 Types of Genomics

5.1.2 Methods for Whole Genome Sequencing

5.2 Proteomics: Concept and Definition

5.2.1 Methods used for Proteome Analysis

5.3 Concept of Bioinformatics

5.3.1 Database and Classification

5.3.2 Data Retrieval Tool

6. Bioremediation

6.1 Introduction and Concept

6.2 Microbial Remediation

6.3  Phytoremediation

7. Biofuel Technology

7.1 Definition, Concept and Types of Renewable and Non-renewable Energy Sources

7.1.1 Definition

7.1.2 Concept

7.1.3 Renewable and Non-renewable Energy Resources