Contents

Plant Tissue Culture

1. Introductory History

1. Concepts of Cell Theory

2. Cellular Totipotency

3. Landmarks in Plant Tissue Culture

4. The General Technique

5. Applications of Plant Tissue Culture

2. Infrastructure and Organization of Plant Tissue Culture Laboratory

1. General and Aseptic Laboratory

2. Different Work Areas

3. Equipments and Instruments used in Plant Tissue Culture

3. Aseptic Techniques

1. Introduction

2. Washing and Preparation of Glassware

3. Packing and Sterilization

4. Aseptic Work Station

5. Precautions to maintain Aseptic Conditions

4. Culture Media

1. Nutritional Requirements of the Explants

2. Plant Growth Regulators (PGR’s) and their In Vitro Roles

3. Preparation of Medium

4. Importance of Mineral Components in the Nutrient Medium

5. ‘Explant’ for Plant Tissue Culture

1. Explant

2. Response of Explants In Vitro – Dedifferentiation and Redifferentiation

2.3 Embryogenesis

6. Callus Culture Technique

1. Introduction

2. Principle

3. Callus Cultures

7. Suspension Culture Technique

1. Introduction

2. Principle

3. Protocol for the Establishment of Suspension Culture

4. Types of Suspension Cultures

5. Measurement of Growth

6. Experiments to assess the Cell Viability

7. Synchronization

8. Importance and Uses of Suspension Culture

8. Organ Culture Technique

1. Root Tip Cultures

2. Leaf culture

3. Shoot Tip Culture

4. Meristem Culture

9. Anther and Polen Culture

1. Anther Culture

2. Pollen Culture

10. Ovary, Ovule, Embryo and Endosperm Culture

1. Ovary Culture

2. Ovule Culture

3. Embryo Culture

4. Endosperm

11. Protoplast

1. Methods of Isolation of Protoplasts

2. Maintenance of Protoplasts

3. Culture of Protoplasts

4. Somatic Hybridization

5. Genetic Transformations

12. Parameters to assess Growth and Development In Vitro

1. Micropropagation (In Vitro Multiplication)

2. Production of Artificial Seeds

3. Parameters to Assess Growth and Development In Vitro 1

13. Somaclonal Variation

1. Introduction

2. Mechanisms underlying Genetic Variation

14. Applications of Plant Tissue Culture

1. Applications of Cell and Tissue Culture

Animal Tissue Culture

1. Introduction to Animal Tissue Culture

1. Introduction

2. In Vivo versus In Vitro Growth Conditions for Cells of Multicellular Organisms

3. Concept of Monolayer, Suspension, Histotypic/ Organotypic, Organ Culture

4. Maintenance of Aseptic Conditions

2. Equipment and Infrastructure

1. Laboratory Design

2. Instruments used in ATC

3. Labware: TC flasks

3. Nutrition and Physiology

1. Rationale behind Medium Formulation

2. Serum and Serum Free Media

3. Balanced Salt Solutions

4. Primary Cell Culture

1. Source Selection

2. Different Methods of Establishing Primary Cell Culture

3. Fibroblast Culture

4. Lymphocytes Culture

5. Cell Lines

1. Evolution of Cell Lines

2. Finite and Transformed Cell Lines

3. Mammalian and Insect Cell Line Growth Conditions

4. Subculture

6. Characterization of Cell Lines

1. Need for Characterization

2. Karyotyping

7. Cell Storage and Distribution

1. Cryopreservation

2. Cell Repositories

8. Application of Animal Cell Cultures

1. Cell Culture Technology Areas