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Microbiology-II

Name: Microbiology-II
SKU: 1831
Availability: InStock

Book ID: 1831

Author: Dr. Avinash Bholay

ISBN: 978-81-944620-7-1

About Author

Dr. Avinash D. Bholay began his teaching career in 1985 at Jankidevi Bajaj Science College, Wardha and served till 1994. Since last fifteen years he is a faculty member and coordinator for the P.G. Microbiology and Biotechnology Department of the K.T.H.M College, Nashik. After completing M.Sc in Microbiology in 1985 with merit from Nagpur University, he joined the project at P. G. Biochemistry and Microbiology Department and National Environmental Engineering Research institute, NEERI, Nagpur, as a research fellow and received his Ph.D degree in 1992. During this period he has also successfully completed D.M.L.T; M.A (Pub. Adm.); D.B.M (Gold Medalist) and LL.B from R.T.M Nagpur University. He has worked as research fellow in V.P. Chest Institute and National Institute of Communicable Diseases at Delhi. During the period 1994-2000, he worked in Citric India Ltd., Nashik, as Fermentation Production Manager and Consultant to industries for Effluent Treatment Plants. He has shouldered the responsibilities of Chairmanship and Coordinatorship of Board of Examinations, S.P. Pune University, Pune, in P.G Microbiology and Biotechnology for many years. He is also a visiting faculty and consulting Microbiologist to many professional institutes of repute. He has published and presented more than seventy five research papers in National and International research journals. He has also guided many M.Phil and Ph.D students as recognized Research Guide in Microbiology and Environmental Science, S.P. Pune University, Pune. He is regular member of various Medical and Scientific associations and societies.

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Description

Contents

1. Cultivation, growth and isolation of microorganisms
A. Microbial Nutrition
1. Nutritional Requirements
2. Environmental Requirements
3. Classification of Nutrients
3.1 Macronutrients
3.2 Micronutrients
4. Modes of Nutrition
5. Nutritional Classification of Bacteria
5.1 Autotrophic Microorganisms
5.2 Heterotrophic Microorganisms
6. Types of Culture Media
6.1 Synthetic Media (Chemically Defined Media)
6.2 Non-synthetic or Complex or Biological Media
6.3 Specific Media
7. Some Microbiological Media
7.1 General Purpose Media for Bacteria
7.2 General Purpose Media for Cyanobacteria (Blue Green Algae)
7.3 General Purpose Media for Fungi
8. Methods to Determine Nutritional Requirements
8.1 Auxanographic Technique
8.2 Replica Plating Technique (Laderberg’s Technique)
8.3 Multipoint Inoculator
B. Microbial Growth
1. Reproduction in Microorganisms
2. Growth Rate and Generation Time
3. Mathematical Expression for Growth
4. The Growth Curve
5. Continuous Culture of Microorganisms
5.1 Chemostat
5.2 Turbidostat
5.3 Dialysis Technique
6. Synchronous Culture
6.1 Selection by Size and Age
6.2 Selection by Induction Technique
7. Microbial Cultures Techniques
7.1 Isolation of Pure Culture
7.2 Maintenance of Pure Culture
7.3 Methods of Preservation
7.4 Systematic Study of Pure Cultures
8. Anaerobic Culture Techniques
8.1 Air Trapping Method
8.2 Phosphorous Stick Method
8.3 Pyrogallol Method
8.4 Anaerobic Chamber Method
9. Measurement of Growth
9.1 Determination of the Number of Cells
9.2 Determination of Cell Mass
9.3 Determination of Cell Activity

2. CONTROL OF MICROBIAL GROWTH
1. Microbial Control
2. Definition of Terms
3. Factors Influencing Antimicrobial Activity
3.1 Factors Related to Killing Agents
3.2 Factors Related to Organisms to be killed
4. Mechanisms of Cell Injury
4.1 Damage to the Cell Wall
4.2 Damage to the Cytoplasmic Membrane
4.3 Denaturation and Coagulation of Protein
4.4 Inhibition of Metabolic Reactions
5. Physical Control Agents
5.1 Heat
5.2 Desiccation
5.3 Osmotic Pressure
5.4 Surface Tension
5.5 Radiations
5.6 Filtration
6. Chemical Control Agents
6.1 Antiseptics and Disinfectants
6.2 Antimetabolites
6.3 Antibiotics
7. Characteristics of an Ideal Disinfectant
8. Evaluation of Disinfectants
9. Testing for the Drug Susceptibility of Microorganisms

3. MICROBIAL INTERACTIONS
1. Microbial Associations
1.1 Microbial Ecological System
1.2 Neutral Associations
1.3 Beneficial Associations
1.4 Detrimental Associations
1.5 Rhizosphere
1.6 Plant Pathogens
1.7 Human Pathogens
2. Handling of Microorganisms and Biosafety Measures
2.1 Biosafety in the Microbiology Laboratory
2.2 Routes of Infection in the Laboratory
2.3 Safety Facilities in Laboratory
2.4 Safety Code for Microbiology Laboratory
2.5 Safe Code of Practice for a Microbiology Laboratory
2.6 Biological Safety Levels
2.7 Aseptic Handling of Microorganisms