Contents

1.    Introduction to Fermentation and Bioprocessing Engineering 

1.    Introduction     

2.    Historical Perspectives

3.    Lay out of a Typical Fermentation Unit   

4.    Bioprocesses     

5.    Components of Bioprocess     

6.    Types of Fermentation  

2.    Microbial Screening and Strain Improvement     

1.    Introduction     

2.    Screening: Definition and Objectives     

3.    Strain Improvement     

4.    Inoculum Development   

3.    Bioreactor Design and Construction 

1.    Introduction     

2.    Bioreactor Design

3.    Design of typical Batch Fermenter  

4.    Aeration System (Sparger) and Agitator Types   

5.    Steam Locks

6.    Additional Accessories 

7.    Safety     

8.    Different Designs of Bioreactors   

4.    Media Constituents and Optimization

1.    Introduction     

2.    Carbon Source    

3.    Nitrogen Source  

4.    Medium Optimization    

5.    Air and Media Sterlization   

1.    Introduction     

2.    Concept of Aseptic Operations and Containment  

3.    Air Sterlization 

4.    Principles of Media Sterilization  

6.    Bioprocess Parameters: Measurements and Control

1.    Introduction     

2.    Physical and Chemical Parameters   

3.    Bioprocess Parameters  

4.    Scale up and Scale down Bioprocess Development 

7.    Downstream Processing  

1.    Introduction     

2.    Precipitation    

3.    Filtration 

4.    Centrifugation   

5.    Cell Disruption  

6.    Liquid –Liquid Extraction    

7.    Chromatography   

8.    Membrane Separation Processes

9.    Drying     

10.   Whole Broth Processing

8.    Bioprocess Products    

1.    Introduction     

2.    Biomass based products 

3.    Enzymes    

4.    Antibiotics

5.    Vitamins   

6.    Amino Acids

7.    Vaccine    

8.    Bio-Transformation Product   

9.    Quality Control and Assurance

1.    Introduction     

2.    Concept of Good Manufacturing Practices (GMP) and Standard …     

3.    Quality Control and Quality Assurances   

4.    Tests used for Quality Assurance of Finished Products

10.   Bioprocess Economics   

1.    Introduction     

2.    Basic Objectives in Developing Economically Viable Process 

3.    Market Potential or Total Addressable Market(TAM)    

4.    Fixed and Variable Cost

5.    Depreciation     

6.    Amortization     

7.    Selection of Pricing