Contents

1.Introduction to Biochemical and Biophysical Techniques   

1.Introduction   

2.Lab Safety     

3.Scientific Notation & Units

4.Errors and Accuracy in experimentation 

5.Understanding of concentration of solution   

6.Acids and Bases

7.Polyprotic Acids     

8.Buffers

2.Microscopy     

1.Introduction

2.Origin of Microscopes

3.Basic Principle of Microscope    

4.The Concept of Resolving Power   

5.Different types of Microscopy    

6.Different types of Microscopes   

7.Preparation of specimens for different types of microscopy

3.Spectrophotometry    

1.Introduction

2.Electromagnetic radiation  

3.Electromagnetic Spectra    

4.Light Absorption and Excitation of Electrons 

5.Beer and Lambert’s Law     

6.Fluorescence Spectroscopy

4.Centrifugation 

1.Introduction

2.Basic Principle of Sedimentation 

3.Types of Centrifugation

4.Density Gradient Centrifuges     

5. Types of Rotors     

6.Care, Maintenance and Safety of Centrifuges

5.Chromatography 

1.Introduction   

2.Principle of Chromatography

3.Types of Chromatography

6.Electrophoresis

1.Introduction   

2.Electrophoresis

3.Electrophoresis of Proteins

4.Electrophoresis of Nucleic Acids