Animal Tissue Culture

Contents

1. Introduction to Animal Culture

1. History and Development of Animal Tissue Culture

2. In Vivo versus In Vitro Growth Conditions for Cells of Multicellular Organisms

2.1 In Vitro Studies

2.2 In Vivo Studies

3. Scientists Contribution in Animal Tissue Culture

2. Basic Concepts in Animal Tissue Culture and Types of Cultures in ATC

1. Primary Cell Culture

2. Secondary Culture

3. Cell Line

4. Organ Culture, Organotypic Culture/Histotypic Culture

4.1 Organ Culture

4.2 Histotypic Culture

4.3 Organotypic Culture

5. Concept of Monolayer Culture/Suspension culture

5.1 Monolayer Cultures

5.2 Suspension Cultures

3. ATC Laboratory and Equipment

1. Laboratory Design

1.1 Basic Requirement for Cell Culture Laboratory

2. Equipments used in ATC

3. Labware: TC flasks

4. Nutrition and Physiology of Cultured Cells

1. Nutritional Requirement and Physiology of Cultured Cells

1.1 Somatomedin and Hormones

1.2 Osmotic Pressure

1.3 pH

2. Principles of Medium Formulation

2.1 Natural Media

2.2 Artificial Media

3. Serum and Serum Free Media

3.1 Serum Containing Media

3.2  Serum-free Media

4. Balanced Salt Solutions

4.1 Examples of Balanced Salt Solutions

4.2 Development of Balanced Salt Solutions

5. Aseptic Condition

1. Maintenance of Aseptic Conditions

2. Contamination, Types of Contaminants

3. Methods of Detection of Contaminants

4. Cross Contamination

5. Primary Cell Culture

1. Introduction

2. Source Selection

2.1  Sterilization of Explant Tissues

2.2 Culture of Explants

2.3 Dissociation Method

3. Different Methods of Establishing Primary Cell Culture

4. Fibroblast Culture

5. Lymphocytes Culture

7. Cell Lines

1. Evolution of Cell Lines

2. Finite and Transformed Cell Lines

3. Mammalian and Insect Cell Line Growth Conditions

3.1  Mammalian Cell Lines

3.2 Insect Cell Lines

4. Subculture

5. Cell Passage Number and Significance

8. Organ Culture

1. Organ Culture and Histotypic Cultures

1.1 Organ Culture

1.2 Histotypic Cultures

1.3 Organotypic Cultures

9. Cell repository and Cell Banks

1. Cryopreservation

1.1 Preparation

1.2 Cryoprotectants

1.3 Cryopreservation Protocol

1.4 Precautions during Cryopreservation

2. Cell Repositories

2.1 Functions of Cell Banks

2.2 Maintenance of Cell Lines

10. Application of Animal Cell Cultures in Different Fields

1. Cell Culture Technology Areas

1.1  Vaccines

1.2  Interferons

1.3 Recombinant Proteins

1.4 Hybrid Antibodies

1.5 Antibodies