Theme Options

NEWSLETTER

Plant and Animal Tissue Culture

BI 1239

New

 

Plant & Animal Tissue Culture

Edition: 4th (2013)

More details

Rs.170

Contents

Plant Tissue Culture

1. Introductory History

1.1 Concepts of Cell Theory  

1.2 Cellular Totipotency     

1.3 Milestones in Plant Tissue Culture   

1.4 The General Technique    

1.4.1 Important Features of Tissue Culture Technique 

1.4.2 Importance of Tissue Culture Technique   

1.5 Applications of Plant Tissue Culture 

2. Infrastructure and Organization of Plant Tissue Laboratory    

2.1 General Requirements of Tissue Culture Laboratory

2.2 Equipments and Instruments used in Plant Tissue Culture

2.3 Different Work Areas     

2.3.1 Culture Room     

2.3.2 Bottle Wash Machine    

2.3.3 Hand and Leg Wash Room 

2.3.4 Dressing Room    

2.3.5 Culture Transfer Room  

3. Aseptic Techniques  

3.1 Introduction 

3.2 Washing and Preparation of Glass Wares     

3.3 Sterilization

3.4 Precautions to Maintain Aseptic Conditions 

4. Culture Medium

4.1 Nutritional Requirements of the Explants   

4.1.1 Composition of M. S. Medium  

4.1.2 Plant Growth Regulators (PGR’s) and their In Vitro Roles   

4.2 Preparation of Medium    

4.3 Importance of Mineral Components in the Nutrient Medium

5. ‘Explant’ For Plant Tissue Culture    

5.1 Explant

5.2 Plant Histology    

5.3 Response of Explants In Vitro - Dedifferentiation & Redifferentiation    

6. Callus Culture Technique  

6.1 Introduction 

6.2 Principle    

6.3 Callus Cultures    

6.3.1 Protocol for the Culture of Plant Tissues

6.3.2 Significance of Callus Culture    

6.3.3 Factors affecting Callus Culture   

6.3.4 Internal Structure of Callus 

7. Suspension Culture Technique    

7.1 Introduction 

7.2 Protocol for the Establishment of Suspension Culture   

7.3 Types of Suspension Cultures   

7.4 Measurement of Growth    

7.4.1 Cell Packing Method    

7.4.2 Direct Cell Count Method     

7.4.3 Direct Weight Method   

7.4.4 Protein Measurement Technique

7.5 Experiments to Assess the Cell Viability   

7.5.1 Evan’s Blue Dye treatment    

7.5.2 Chemical Method  

7.6 Synchronization    

7.7 Importance and Uses of Suspension Culture  

8. Organ Culture Technique   

8.1 Shoot Tip Culture  

8.2 Root Tip Cultures  

8.3 Leaf Culture 

8.4 Meristem Culture   

9. Anther and Pollen Culture Technique   

9.1 Anther Culture     

9.2 Pollen Culture     

9.2.1 Protocol for Pollen Culture Technique    

9.2.2 Advantages of Pollen Culture over Anther Culture     

9.2.3 Pollen Biotechnology   

10. Ovary, Ovule, Embryo and Endosperm Culture 

10.1 Ovary Culture     

10.2 Ovule Culture     

10.3 Embryo Culture    

10.4 Endosperm   

11. Protoplast   

11.1 Methods of Isolation of Protoplasts 

11.2 Maintenance of Protoplasts    

11.3 Culture of Protoplasts  

11.3.1 Viability Tests for Protoplasts   

11.3.2 Regeneration of Cell Wall   

11.3.3 Cell Division and Callus Formation

11.3.4 Importance of Protoplast Culture  

11.4 Somatic Hybridization   

11.4.1 Protoplast Fusion     

11.4.2 Selection of Hybrid Protoplasts   

11.4.3 Regeneration of Plantlets   

11.4.4 Production of Symmetric and Asymmetric Hybrids

11.4.5 Cybrids   

11.5 Genetic Transformations 

11.5.1 Gene Manipulation in Plants 

11.5.2 Direct DNA Transfer Methods 

11.5.3 Transformation of Protoplasts     

11.5.4 Agrobacterium Mediated Gene Transfer    

11.5.5 Integration and Expression of Foreign DNA

11.5.6 Applications in Crop Improvement  

12. Different Routes of Multiplication In Vitro

12.1 Micropropagation (In Vitro Multiplication)

12.2 Methods of Micropropagation   

12.2.1 Proliferation of Auxiliary Buds   

12.2.2 Induction of Adventitious Buds, Bulbs and Protocorms

12.2.3 Somatic Embryogenesis 

12.2.4 Meristem Culture

12.3 Applications

12.4 Limitation  

12.5 Production of Artificial Seeds

12.5.1 Techniques to Produce Artificial Seeds  

12.5.2 Types of Artificial Seeds   

12.5.3 Salient Features of Artificial Seeds    

13. Somaclonal Variation     

13.1 Introduction

13.1.1 Terminology     

13.1.2 Origin of Somaclonal Variation    

13.2 Isolation of Somaclonal Variants    

13.2.1 Advantages

13.2.2 Limitations     

13.3 Germplasm Preservation  

13.4 Mechanisms Underlying Genetic Variation   

14. Introduction To Secondary Metabolite production In Vitro Biotransformations    

14.1 Introduction

14.2 Culture of Plant Cells for the Extraction of Secondary Metabolism

14.3 Commercial Aspects

14.4 Production of Callus for the Extraction of Secondary Metabolites  

14.5 Culture Adjustments for the Extraction of Specific Substances     

14.6 Biotransformation in Plants   

14.6.1 Elicitor - Dependent Biosynthesis 

14.6.2 Immobilization of Plant Cells     

14.7 Optimisation of the Yield     

14.8 Applications

14.9 Advantages and Limitations    

Animal tissue culture

1. Animal Tissue Culture     

1.1 Introduction 

1.2 Basic Requirement for Cell Culture Laboratory    

1.3 Source of Explant Tissues

1.4 Collection of Explants

1.5 Precautions  

1.6 Maintainance of Aseptic Condition in Cell Culture Laboratory 

2. NUTRITION and Physiology Media Components   

2.1 Introduction 

2.1.1 Natural Media    

2.1.2 Artificial Media 

2.2 Preparation and Sterilization of Substrate and Medium  

3. Primary Cell Culture

3.1 Introduction 

3.1.1 Sterilization of Explant Tissues   

3.1.2 Culture of Explants    

3.1.3 Dissociation Method    

3.2 lymphocytes  

4. Cell Lines    

4.1 Introduction 

4.2 Immortalization of Cell Lines  

4.3 Cell Line Designations   

4.4 Selection of Cell Line   

4.5 Routine Maintenance

4.6 Replacement of Medium    

4.7 Animal Cell Subculture   

4.7.1 Criteria for Subculture

4.7.2 Examples of Cell Lines 

4.8 Insect Cell Culture

4.8.1 Applications     

4.8.2 Method     

5. Organ and Tissue Culture  

5.1 Organ Culture

5.1.1 Technique  

5.1.2 Advantages 

5.1.3 Limitations

5.1.4 Applications

5.2 Tissue Culture

6. Karyotyping   

6.1 Definition   

6.2 Chromosomal Analysis (Karyotyping) from the appropriate Sample

6.2.1 Human Karyotype  

6.2.2 Characterization 

6.3 Chromosome Staining

6.4 Banding Technique  

6.4.1 Conventional Staining  

6.4.2 G Bands by Trypsin using Giemsa (GTG) Banding  

6.4.3 C Bands using Barium Hydroxide Giemsa (CBG) Banding  

6.4.4 Silver Staining of Nucleolar Organiser Regions (AgNOR)     

6.4.5 Q Bands by Fluorescence using Quinacrine (QFQ) 

6.5 Applications of Karyotyping    

6.6 Some Examples of Karyotypes    

6.6.1 Klinefelter Syndrome   

6.6.2 Turner’s Syndrome

7. Cell Repositories  and their Function 

7.1 Introduction 

7.2 Functions of Cell Banks  

7.3 Maintenance of Cell Lines

7.4 Precautions during Cryopreservation  

8. Application of Animal Cell Culture    

8.1 Vaccines     

8.2 Interferons  

8.3 Recombinant Proteins     

8.4 Hybrid Antibodies  

8.5 Antibodies   

8.5.1 Production of Monoclonal Antibodies

8.5.2 Applications of Monoclonal Antibodies    

 

No customer comments for the moment.

Write a review

Write a review

9 other products in the same category:

Customers who bought this product also bought: