Contents

1. Introduction To Industrial Microbiology

1. Concept of Fermentation Process

2. Typical Layout of a Typical Fermentation Unit

2.1 Types of Fermentation

2. Screening and Strain Improvement Techniques

1. Isolation and Screening of Industrially Important Microorganisms

1.1 Screening: Definition and Objectives

2. Primary and Secondary Screening with Suitable Example

2.1 Primary Screening

2.2 Secondary Screening

3. Strain Improvement – Objectives

3.1 Methods for Strain Improvement

3.2  Auxotrophic Mutants

3.3 Analogue Resistant

3. Bioreactor Design

1. Characteristics of Bioreactor/Ideal Fermentor

2. Construction Materials Used, Surface Treatment of the Material

2.1 Construction Materials Used

2.2 Surface Treatment of the Material

3. Design of Typical Batch Bioreactor/Fermenter

3.1 Aeration System (Sparger) and Agitator Types

3.2 Baffles

3.3 Seals and Valves Used

3.4 Valves

3.5 Steam Traps

4. Different Designs of Bioreactors

4.1 Air Lift

4.2 Packed Bed Bioreactors

4.3 Fluidized Bed Reactor

4. Large Scale Media and Sterilization

1. Media Components and Optimization

1.1 Carbon Source

1.2 Nitrogen Source

1.3 Buffering Agents

1.4 Concept of Medium Optimization

2. Media Sterilization

2.1 Principles

2.2 Equipment used in Sterilization

3. Air Sterilization

3.1 Principle of Air Sterilization

5. Measurement and Control of Different Bioprocess Parameters

1. Temperature

2. pH Measurement

3. Foam

4. Dissolved Oxygen

5. Microbial Biomass

6. Concept of Scale-up Studies and Scale-down

6. Downstream Processing of fermentation Product – Methods and Equipments

1. Definition: Unit Operations and Downstream Processing

a. Precipitation: Agents used are Salts, Organic Solvent

b. Filtration

c. Centrifugation

d. Cell Disruption

e. Solvent Extraction–Liquid-Liquid Extraction

f. Drying

7. Large Scale Manufacturing Process

1. Baker’s Yeast

2. Citric Acid Production

3. Vitamin B12

4. L-Lysine

5. Alcohol

6. Enzymes