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Recombinant DNA Technology

1684

New

According to New Revised Syllabus w.e.f. 2015-16

Recombinant DNA Technology

Author: Dr. Shankar Laware, Sangita Sontakke

Book ID: 1684

ISBN: 978-93-5016-367-2

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Rs.200

Contents

1. Milestones of Genetic Engineering – Historical Perspective     

1. History of Biotechnology   

2. The Growing Significance of Biotechnology    

3. Scope and Importance of Genetic Engineering  

4. Agriculture and Gene Technology  

5. Plant Transformation 

6. Marker Assisted Selection  

7. Challenges in Product Development using Gene Technology  

8. Gene Therapy   

9. Biotechnology and Intellectual Property Rights     

10.Advantages of Biotechnologies   

2. Molecular Tools and Applications 

1. Introduction   

2. Restriction Enzymes  

3. DNA Ligases    

4. Polymerases    

5. Alkaline Phosphatase 

3. Gene Cloning Vehicles

1. Introduction   

2. Types of Vectors     

3. Properties of a Good Vector

4. Plasmids 

5. Phage    

6 Additional Vectors   

7. Host     

4. Transformation

1. Introduction   

2. Techniques of Introducing DNA    

3. Selection and Screening of Recombinant Clones

4. Identification or Selection of Specific Clone

5. Nucleic Acid Purification 

1. Introduction   

2. Extraction and Isolation of RNA  

3. Purification of Nucleic Acids    

4. Purification of RNA  

5. Yield and Yield Analyses of Nucleic Acids    

6. Isolation of Plasmid DNA   

6. DNA Sequencing Techniques  

1. Introduction   

2. Maxam and Gilbert’s Chemical Degradation Method    

3. Sanger’s Dideoxy method    

4. Automated DNA Sequencing   

5. Next Generation Sequencing 

7. Restriction Enzyme Digestion and Restriction Mapping

1. Introduction   

2. Restriction Enzyme Digestion     

3. Restriction Mapping  

4. Southern Blotting and Analysis   

5. Autoradiography

6. Northern Blotting    

7. Western Blotting     

8. Genomic Libraries and Screening of Recombinants    

1. Introduction   

2. Screening of Recombinants  

3. In Situ Hybridization

4. Probe Labeling and Detection     

9. Gene Manipulations by site-directed mutagenesis-PCR Technology 

1. Introduction   

3. General Approach     

4. PCR - Based in Vitro Mutagenesis 

5 Applications of Site-directed Mutagenesis    

6. Gene Library   

7. CDNA Library Construction  

8. Comparison between CDNA Library And Genomic Library

10.   Genome Mapping   

1. Introduction   

2. Genome Maps    

3. Molecular Markers    

4. Techniques for Genome Mapping    

5. DNA Fingerprinting   

6. Uses Of DNA Fingerprinting 

11.Applications of Genetic Engineering   

1. Introduction   

2. Application of Genetic Engineering In Animals

3. Application Of Genetic Engineering In Plants 

4. Applications Of Genetic Engineering In Microbes    

5. Recombinant DNA Guidelines

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